Probing Protein Secondary Structure using EPR: Investigating a Dynamic Region of Visual Arrestin.

ABSTRACT: One key application of site-directed spin labeling (SDSL) electron paramagnetic resonance (EPR) spectroscopy is the determination of sequence-specific secondary structure in proteins. Regular secondary structure leads to a periodic variation in both side chain motion and solvent accessibility, two properties easily monitored by EPR techniques. Specifically, saturation recovery (SR) EPR spectroscopy has proven to be useful for making accessibility measurements for multiple protein structure populations by determining individual accessibilities and is therefore well suited to study the structure of proteins exhibiting multiple conformations in equilibrium. Here we employ both continuous wave and SR EPR spectroscopy in combination to examine the secondary structure of a short sequence showing

SUBMITTER: Francis DJ 

PROVIDER: S-EPMC4240029 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature