A Raman microspectroscopy study of water and trehalose in spin-dried cells.

ABSTRACT: Long-term storage of desiccated nucleated mammalian cells at ambient temperature may be accomplished in a stable glassy state, which can be achieved by removal of water from the biological sample in the presence of glass-forming agents including trehalose. The stability of the glass may be compromised due to a nonuniform distribution of residual water and trehalose within and around the desiccated cells. Thus, quantification of water and trehalose contents at the single-cell level is critical for predicting the glass formation and stability for dry storage. Using Raman microspectroscopy, we estimated the trehalose and residual water contents in the microenvironment of spin-dried cells. Individual cells with or without intracellular trehalose were embedded in a solid thin layer of extracell

SUBMITTER: Abazari A 

PROVIDER: S-EPMC4241435 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature