Memory B-cell and antibody responses induced by Plasmodium falciparum sporozoite immunization.

ABSTRACT:

Background

Immunization of healthy volunteers during receipt of chemoprophylaxis with Plasmodium falciparum sporozoites (CPS-immunization) induces sterile protection from malaria. Antibody responses have long been known to contribute to naturally acquired immunity against malaria, but their association with sterile protection after whole sporozoite immunization is not well established. We therefore studied the induction and kinetics of malaria parasite antigen-specific antibodies and memory B-cells (MBCs) during CPS-immunization and their correlation with protection from challenge infection.

Methods

We assessed humoral reactivity to 9 antigens representing different stages of the life cycle of P. falciparum by performing standardized MBC enzyme-linked immunospot and enzyme-l

SUBMITTER: Nahrendorf W 

PROVIDER: S-EPMC4241945 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature