Sugar-responsive gene expression, invertase activity, and senescence in aborting maize ovaries at low water potentials.

ABSTRACT:

Background and aims

Ovary abortion can occur in maize (Zea mays) if water deficits lower the water potential (psiw) sufficiently to inhibit photosynthesis around the time of pollination. The abortion decreases kernel number. The present work explored the activity of ovary acid invertases and their genes, together with other genes for sucrose-processing enzymes, when this kind of abortion occurred. Cytological evidence suggested that senescence may have been initiated after 2 or 3 d of low psiw, and the expression of some likely senescence genes was also determined.

Methods

Ovary abortion was assessed at kernel maturity. Acid invertase activities were localized in vivo and in situ. Time courses for mRNA abundance were measured with real time PCR. Sucrose was fed to the stems

SUBMITTER: McLaughlin JE 

PROVIDER: S-EPMC4242214 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature