Molecular characterization of serous ovarian carcinoma using a multigene next generation sequencing cancer panel approach.

ABSTRACT:

Background

High grade serous ovarian cancer is one of the poorly characterized malignancies. This study aimed to elucidate the mutational events in Malaysian patients with high grade serous ovarian cancer by performing targeted sequencing on 50 cancer hotspot genes.

Results

Nine high grade serous ovarian carcinoma samples and ten normal ovarian tissues were obtained from Universiti Kebangsaan Malaysia Medical Center (UKMMC) and the Kajang Hospital. The Ion AmpliSeq™ Cancer Hotspot Panel v2 targeting "mutation-hotspot region" in 50 most common cancer-associated genes was utilized. A total of 20 variants were identified in 12 genes. Eleven (55%) were silent alterations and nine (45%) were missense mutations. Six of the nine missense mutations were predicted to be deleterious w

SUBMITTER: Ab Mutalib NS 

PROVIDER: S-EPMC4242548 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature