Increased tumor distribution and expression of histidine-rich plasmid polyplexes.

ABSTRACT:

Background

Selecting nonviral carriers for in vivo gene delivery is often dependent on determining the optimal carriers from transfection assays in vitro. The rationale behind this in vitro strategy is to cast a net sufficiently wide to identify the few effective carriers of plasmids for in vivo studies. Nevertheless, many effective in vivo carriers may be overlooked by this strategy because of the marked differences between in vitro and in vivo assays.

Methods

After solid-phase synthesis of linear and branched histidine/lysine (HK) peptides, the two peptide carriers were compared for their ability to transfect MDA-MB-435 tumor cells in vitro and then in vivo.

Results

By contrast to their transfection activity in vitro, the linear H2K carrier of plasmids was far more

SUBMITTER: Leng Q 

PROVIDER: S-EPMC4242722 | biostudies-literature | 2014 Sep-Oct

REPOSITORIES: biostudies-literature