Genomic mapping of the MHC transactivator CIITA using an integrated ChIP-seq and genetical genomics approach.

ABSTRACT:

Background

The master transactivator CIITA is essential to the regulation of Major Histocompatibility Complex (MHC)class II genes and an effective immune response. CIITA is known to modulate a small number of non-MHC genes involved in antigen presentation such as CD74 and B2M but its broader genome-wide function and relationship with underlying genetic diversity has not been resolved.

Results

We report the first genome-wide ChIP-seq map for CIITA and complement this by mapping inter-individual variation in CIITA expression as a quantitative trait. We analyse CIITA recruitment for pathophysiologically relevant primary human B cells and monocytes, resting and treated with interferon-gamma, in the context of the epigenomic regulatory landscape and DNA-binding proteins associate

SUBMITTER: Wong D 

PROVIDER: S-EPMC4243378 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature