DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors.

ABSTRACT:

Background

DNA barcoding assumes that a biological entity is completely separated from its closest relatives by a barcoding gap, which means that intraspecific genetic distance (from COI sequences) should never be greater than interspecific distances. We investigated the applicability of this strategy in identifying species of the genus Triatoma from South America.

Findings

We calculated intra and interspecific Kimura-2-parameter distances between species from the infestans, matogrossensis, sordida and rubrovaria subcomplexes. In every subcomplex examined we observed at least one intraspecific distance greater than interspecific distances.

Conclusions

Although DNA barcoding is a straightforward approach, it was not applicable for identifying Southern American Triatoma

SUBMITTER: Justi SA 

PROVIDER: S-EPMC4243934 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature