Expression of genes related to prostaglandin synthesis or signaling in human subcutaneous and omental adipose tissue: depot differences and modulation by adipogenesis.

ABSTRACT:

Objectives

(1) To examine depot-specific PGE2 and PGF2α release and mRNA expression of enzymes or receptors involved in PG synthesis or signaling in human adipose tissues; (2) to identify changes in expression of these transcripts through preadipocyte differentiation; and (3) to examine associations between adipose tissue mRNA expression of these transcripts and adiposity measurements.

Methods

Fat samples were obtained surgically in women. PGE2 and PGF2α release by preadipocytes and adipose tissue explants was measured. Expression levels of mRNA coding for enzymes or receptors involved in PG synthesis or signaling were measured by RT-PCR.

Results

Cultured preadipocytes and explants from omental fat released more PGE2 and PGF2α than those from the subcutaneous depot an

SUBMITTER: Michaud A 

PROVIDER: S-EPMC4244696 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature