Quantum dot-based multiphoton fluorescent pipettes for targeted neuronal electrophysiology.

ABSTRACT: Targeting visually identified neurons for electrophysiological recording is a fundamental neuroscience technique; however, its potential is hampered by poor visualization of pipette tips in deep brain tissue. We describe quantum dot-coated glass pipettes that provide strong two-photon contrast at deeper penetration depths than those achievable with current methods. We demonstrated the pipettes' utility in targeted patch-clamp recording experiments and single-cell electroporation of identified rat and mouse neurons in vitro and in vivo.

SUBMITTER: Andrasfalvy BK 

PROVIDER: S-EPMC4245189 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature