Polyketide synthases in the microbiome of the marine sponge Plakortis halichondrioides: a metagenomic update.

ABSTRACT: Sponge-associated microorganisms are able to assemble the complex machinery for the production of secondary metabolites such as polyketides, the most important class of marine natural products from a drug discovery perspective. A comprehensive overview of polyketide biosynthetic genes of the sponge Plakortis halichondrioides and its symbionts was obtained in the present study by massively parallel 454 pyrosequencing of complex and heterogeneous PCR (Polymerase Chain Reaction) products amplified from the metagenomic DNA of a specimen of P. halichondrioides collected in the Caribbean Sea. This was accompanied by a survey of the bacterial diversity within the sponge. In line with previous studies, sequences belonging to supA and swfA, two widespread sponge-specific groups of polyketide syntha

SUBMITTER: Della Sala G 

PROVIDER: S-EPMC4245539 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature