The spatial effect of protein deuteration on nitroxide spin-label relaxation: implications for EPR distance measurement.

ABSTRACT: Pulsed electron-electron double resonance (PELDOR) coupled with site-directed spin labeling is a powerful technique for the elucidation of protein or nucleic acid, macromolecular structure and interactions. The intrinsic high sensitivity of electron paramagnetic resonance enables measurement on small quantities of bio-macromolecules, however short relaxation times impose a limit on the sensitivity and size of distances that can be measured using this technique. The persistence of the electron spin-echo, in the PELDOR experiment, is one of the most crucial limitations to distance measurement. At a temperature of around 50 K one of the predominant factors affecting persistence of an echo, and as such, the sensitivity and measurable distance between spin labels, is the electron spin echo deph

SUBMITTER: El Mkami H 

PROVIDER: S-EPMC4245719 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature