RNA-sequencing reveals early, dynamic transcriptome changes in the corollas of pollinated petunias.

ABSTRACT:

Background

Pollination reduces flower longevity in many angiosperms by accelerating corolla senescence. This response requires hormone signaling between the floral organs and results in the degradation of macromolecules and organelles within the petals to allow for nutrient remobilization to developing seeds. To investigate early pollination-induced changes in petal gene expression, we utilized high-throughput sequencing to identify transcripts that were differentially expressed between corollas of pollinated Petunia × hybrida flowers and their unpollinated controls at 12, 18, and 24 hours after opening.

Results

In total, close to 0.5 billion Illumina 101 bp reads were generated, de novo assembled, and annotated, resulting in an EST library of approximately 33 K genes. Over

SUBMITTER: Broderick SR 

PROVIDER: S-EPMC4245787 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature