Molecular characterization of H3N2 influenza A viruses isolated from Ontario swine in 2011 and 2012.

ABSTRACT:

Background

Data about molecular diversity of commonly circulating type A influenza viruses in Ontario swine are scarce. Yet, this information is essential for surveillance of animal and public health, vaccine updates, and for understanding virus evolution and its large-scale spread.

Methods

The study population consisted of 21 swine herds with clinical problems due to respiratory disease. Nasal swabs from individual pigs were collected and tested by virus isolation in MDCK cells and by rtRT-PCR. All eight segments of 10 H3N2 viruses were sequenced using high-throughput sequencing and molecularly characterized.

Results

Within-herd prevalence ranged between 2 and 100%. Structurally, Ontario H3N2 viruses could be classified into three different groups. Group 1 was the mo

SUBMITTER: Grgic H 

PROVIDER: S-EPMC4245826 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature