A platform for rapid prototyping of synthetic gene networks in mammalian cells.

ABSTRACT: Mammalian synthetic biology may provide novel therapeutic strategies, help decipher new paths for drug discovery and facilitate synthesis of valuable molecules. Yet, our capacity to genetically program cells is currently hampered by the lack of efficient approaches to streamline the design, construction and screening of synthetic gene networks. To address this problem, here we present a framework for modular and combinatorial assembly of functional (multi)gene expression vectors and their efficient and specific targeted integration into a well-defined chromosomal context in mammalian cells. We demonstrate the potential of this framework by assembling and integrating different functional mammalian regulatory networks including the largest gene circuit built and chromosomally integrated to d

SUBMITTER: Duportet X 

PROVIDER: S-EPMC4245948 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature