A portable BRCA1-HAC (human artificial chromosome) module for analysis of BRCA1 tumor suppressor function.

ABSTRACT: BRCA1 is involved in many disparate cellular functions, including DNA damage repair, cell-cycle checkpoint activation, gene transcriptional regulation, DNA replication, centrosome function and others. The majority of evidence strongly favors the maintenance of genomic integrity as a principal tumor suppressor activity of BRCA1. At the same time some functional aspects of BRCA1 are not fully understood. Here, a HAC (human artificial chromosome) module with a regulated centromere was constructed for delivery and expression of the 90 kb genomic copy of the BRCA1 gene into BRCA1-deficient human cells. A battery of functional tests was carried out to demonstrate functionality of the exogenous BRCA1. In separate experiments, we investigated the role of BRCA1 in maintenance of heterochromatin int

SUBMITTER: Kononenko AV 

PROVIDER: S-EPMC4245969 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature