The Ebola virus matrix protein VP40 selectively induces vesiculation from phosphatidylserine-enriched membranes.

ABSTRACT: Ebola virus is from the Filoviridae family of viruses and is one of the most virulent pathogens known with ∼ 60% clinical fatality. The Ebola virus negative sense RNA genome encodes seven proteins including viral matrix protein 40 (VP40), which is the most abundant protein found in the virions. Within infected cells VP40 localizes at the inner leaflet of the plasma membrane (PM), binds lipids, and regulates formation of new virus particles. Expression of VP40 in mammalian cells is sufficient to form virus-like particles that are nearly indistinguishable from the authentic virions. However, how VP40 interacts with the PM and forms virus-like particles is for the most part unknown. To investigate VP40 lipid specificity in a model of viral egress we employed giant unilamellar vesicles with di

SUBMITTER: Soni SP 

PROVIDER: S-EPMC4246110 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature