Assessing proteinase K resistance of fish prion proteins in a scrapie-infected mouse neuroblastoma cell line.

ABSTRACT: The key event in prion pathogenesis is the structural conversion of the normal cellular protein, PrP(C), into an aberrant and partially proteinase K resistant isoform, PrP(Sc). Since the minimum requirement for a prion disease phenotype is the expression of endogenous PrP in the host, species carrying orthologue prion genes, such as fish, could in theory support prion pathogenesis. Our previous work has demonstrated the development of abnormal protein deposition in sea bream brain, following oral challenge of the fish with natural prion infectious material. In this study, we used a prion-infected mouse neuroblastoma cell line for the expression of three different mature fish PrP proteins and the evaluation of the resistance of the exogenously expressed proteins to proteinase K treatment (P

SUBMITTER: Salta E 

PROVIDER: S-EPMC4246229 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature