CD137 signaling enhances tight junction resistance in intestinal epithelial cells.

ABSTRACT: Treatment of Caco-2-BBe intestinal epithelial cells (BBe) with TNF-α and lymphotoxin-β (LT-β) receptor agonists induced the expression of the TNF receptor superfamily gene TNFRSF9/CD137. In the gut, these cytokines are known to be involved in both inflammatory responses and development of organized lymphoid tissues; thus, it was notable that in CD137-deficient mice Peyer's patch M cells lacked transcytosis function. To examine the direct effect of CD137 expression on epithelial cell function independent of other cytokine effects including CD137L triggering, we stably transfected BBe cells to express CD137. CD137 was found at the cell surface as well as the cytoplasm, and confocal microscopy suggested that aggregates of CD137 at the lateral and basolateral surface may be associated with cyt

SUBMITTER: Gusti V 

PROVIDER: S-EPMC4246582 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature