Genome-wide analysis of transcript abundance and translation in Arabidopsis seedlings subjected to oxygen deprivation.

ABSTRACT:

Background and aims

DNA microarrays allow comprehensive estimation of total cellular mRNA levels but are also amenable to studies of other mRNA populations, such as mRNAs in translation complexes (polysomes). The aim of this study was to evaluate the role of translational regulation in response to oxygen deprivation (hypoxia).

Methods

Alterations in total cellular and large polysome (>or=five ribosomes per mRNA) mRNA levels were monitored in response to 12 h of hypoxia stress in seedlings of Arabidopsis thaliana with a full-genome oligonucleotide microarray.

Key results

Comparison of two mRNA populations revealed considerable modulation of mRNA accumulation and diversity in translation in response to hypoxia. Consistent with the global decrease in protein synthesis, h

SUBMITTER: Branco-Price C 

PROVIDER: S-EPMC4247032 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature