Analysis of stranded information using an automated procedure for strand specific RNA sequencing.

ABSTRACT:

Background

Strand specific RNA sequencing is rapidly replacing conventional cDNA sequencing as an approach for assessing information about the transcriptome. Alongside improved laboratory protocols the development of bioinformatical tools is steadily progressing. In the current procedure the Illumina TruSeq library preparation kit is used, along with additional reagents, to make stranded libraries in an automated fashion which are then sequenced on Illumina HiSeq 2000. By the use of freely available bioinformatical tools we show, through quality metrics, that the protocol is robust and reproducible. We further highlight the practicality of strand specific libraries by comparing expression of strand specific libraries to non-stranded libraries, by looking at known antisense transcri

SUBMITTER: Sigurgeirsson B 

PROVIDER: S-EPMC4247151 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature