Genome wide screening of RNAi factors of Sf21 cells reveal several novel pathway associated proteins.

ABSTRACT:

Background

RNA interference (RNAi) leads to sequence specific knock-down of gene expression and has emerged as an important tool to analyse gene functions, pathway analysis and gene therapy. Although RNAi is a conserved cellular process involving common elements and factors, species-specific differences have been observed among different eukaryotes. Identification of components for RNAi pathway is pursued intensively and successful genome-wide screens have been performed for components of RNAi pathways in various organisms. Functional comparative genomics analysis offers evolutionary insight that forms basis of discoveries of novel RNAi-factors within related organisms. Keeping in view the academic and commercial utility of insect derived cell-line from Spodoptera frugiperda, we pu

SUBMITTER: Ghosh S 

PROVIDER: S-EPMC4247154 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature