Primary transcriptome map of the hyperthermophilic archaeon Thermococcus kodakarensis.

ABSTRACT:

Background

Prokaryotes have relatively small genomes, densely-packed with protein-encoding sequences. RNA sequencing has, however, revealed surprisingly complex transcriptomes and here we report the transcripts present in the model hyperthermophilic Archaeon, Thermococcus kodakarensis, under different physiological conditions.

Results

Sequencing cDNA libraries, generated from RNA isolated from cells under different growth and metabolic conditions has identified >2,700 sites of transcription initiation, established a genome-wide map of transcripts, and consensus sequences for transcription initiation and post-transcription regulatory elements. The primary transcription start sites (TSS) upstream of 1,254 annotated genes, plus 644 primary TSS and their promoters within genes,

SUBMITTER: Jager D 

PROVIDER: S-EPMC4247193 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature