Analysis of microRNA transcription and post-transcriptional processing by Dicer in the context of CHO cell proliferation.

ABSTRACT: CHO cells are the mammalian cell line of choice for recombinant production of therapeutic proteins. However, their low rate of proliferation limits obtainable space-time yields due to inefficient biomass accumulation. We set out to correlate microRNA transcription to cell-specific growth-rate by microarray analysis of 5 CHO suspension cell lines with low to high specific growth rates. Global microRNA expression analysis and Pearson correlation studies showed that mature microRNA transcript levels are predominately up-regulated in a state of fast proliferation (46 positively correlated, 17 negatively correlated). To further validate this observation, the expression of three genes that are central to microRNA biogenesis (Dicer, Drosha and Dgcr8) was analyzed. The expression of Dicer, which m

SUBMITTER: Hackl M 

PROVIDER: S-EPMC4247382 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature