Proteome-wide analysis reveals an age-associated cellular phenotype of in situ aged human fibroblasts.

ABSTRACT: We analyzed an ex vivo model of in situ aged human dermal fibroblasts, obtained from 15 adult healthy donors from three different age groups using an unbiased quantitative proteome-wide approach applying label-free mass spectrometry. Thereby, we identified 2409 proteins, including 43 proteins with an age-associated abundance change. Most of the differentially abundant proteins have not been described in the context of fibroblasts' aging before, but the deduced biological processes confirmed known hallmarks of aging and led to a consistent picture of eight biological categories involved in fibroblast aging, namely proteostasis, cell cycle and proliferation, development and differentiation, cell death, cell organization and cytoskeleton, response to stress, cell communication and signal tran

SUBMITTER: Waldera-Lupa DM 

PROVIDER: S-EPMC4247387 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature