Measurement of refractive index by nanoparticle tracking analysis reveals heterogeneity in extracellular vesicles.

ABSTRACT:

Introduction

Optical techniques are routinely used to size and count extracellular vesicles (EV). For comparison of data from different methods and laboratories, suitable calibrators are essential. A suitable calibrator must have a refractive index (RI) as close to that of EV as possible but the RI of EV is currently unknown. To measure EV, RI requires accurate knowledge of size and light scattering. These are difficult to measure as most EVs cannot be resolved by light microscopy and their diameter is smaller than the wavelength of visible light. However, nanoparticle tracking analysis (NTA) provides both size and relative light scattering intensity (rLSI) values. We therefore sought to determine whether it was possible to use NTA to measure the RI of individual EVs.

Methods

SUBMITTER: Gardiner C 

PROVIDER: S-EPMC4247498 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature