Contextualised urinary biomarker analysis facilitates diagnosis of paediatric obstructive sleep apnoea.

ABSTRACT:

Background

Intrinsic variance of the urine proteome limits the discriminative power of proteomic analysis and complicates potential biomarker detection in the context of paediatric sleep disorders.

Methods and results

Using a rigorous workflow for proteomic analysis of urine, we demonstrate that gender and diurnal effects constitute two important sources of variability in healthy children. In the context of disease, complex pathophysiological perturbations magnify these proteomic differences and therefore require contextualised biomarker analysis. Indeed, by performing biomarker discovery in a gender- and diurnal-dependent manner, we identified ∼30-fold more candidate biomarkers of paediatric obstructive sleep apnoea (OSA), a highly prevalent condition in children characteri

SUBMITTER: Becker L 

PROVIDER: S-EPMC4247538 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature