Omics profiles used to evaluate the gene expression of Exiguobacterium antarcticum B7 during cold adaptation.

ABSTRACT:

Background

Exiguobacterium antarcticum strain B7 is a Gram-positive psychrotrophic bacterial species isolated in Antarctica. Although this bacteria has been poorly studied, its genome has already been sequenced. Therefore, it is an appropriate model for the study of thermal adaptation. In the present study, we analyzed the transcriptomes and proteomes of E. antarcticum B7 grown at 0°C and 37°C by SOLiD RNA-Seq, Ion Torrent RNA-Seq and two-dimensional difference gel electrophoresis tandem mass spectrometry (2D-DIGE-MS/MS).

Results

We found expression of 2,058 transcripts in all replicates from both platforms and differential expression of 564 genes (absolute log2FC≥1, P-value<0.001) comparing the two temperatures by RNA-Seq. A total of 73 spots were differentially expressed b

SUBMITTER: Dall'Agnol HP 

PROVIDER: S-EPMC4247613 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature