Dataset Information
Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats.
Ontology highlight
ABSTRACT: Background
In the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N = 2297) and Danish (N = 134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR (RT-qPCR).Findings
In total, 15 RT-qPCR positive swabs were detected. Remarkably, sequencing of positive samples did not confirm the presence of bat associated lyssaviruses but revealed nine distinct novel rhabdovirus-related sequences.Conclusions
Several novel rhabdovirus-related sequences were detected both in German and Danish insectivorous bats. The results also prove that the novel generic pan-lyssavirus RT-qPCR offers a very broad detection range that allows the collection of further valuable data concerning the broad and complex diversity within the family Rhabdoviridae.
SUBMITTER: Fischer M
PROVIDER: S-EPMC4247638 | biostudies-literature | 2014 Nov
REPOSITORIES: biostudies-literature
Publications
Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats.
Virology journal 20141125
<h4>Background</h4>In the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N = 2297) and Danish (N = 134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR (RT-qPCR).<h4>Findings</h4>In total, 15 RT-qPCR positive swabs were detected. Remarkably, sequencing of positive samples did not confirm the presence of bat associated lyssaviruses but revealed nine distinct novel rhabdovirus-related sequenc ...[more]
