Differential expression of the nuclear-encoded mitochondrial transcriptome in pediatric septic shock.

ABSTRACT:

Introduction

Increasing evidence supports a role for mitochondrial dysfunction in organ injury and immune dysregulation in sepsis. Although differential expression of mitochondrial genes in blood cells has been reported for several diseases in which bioenergetic failure is a postulated mechanism, there are no data about the blood cell mitochondrial transcriptome in pediatric sepsis.

Methods

We conducted a focused analysis using a multicenter genome-wide expression database of 180 children ≤ 10 years of age with septic shock and 53 healthy controls. Using total RNA isolated from whole blood within 24 hours of PICU admission for septic shock, we evaluated 296 nuclear-encoded mitochondrial genes using a false discovery rate of 1%. A series of bioinformatic approaches were appli

SUBMITTER: Weiss SL 

PROVIDER: S-EPMC4247726 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature