Expression of α4, αv, β1 and β3 integrins during the implantation window on blastocyst of a mouse model of polycystic ovarian syndromes.

ABSTRACT:

Background

It has been hypothesized that blastocyst integrin expression changes can affect the spontaneous miscarriage in polycystic ovarian syndromes (PCOS).

Objective

In this study, the profile of integrin genes and proteins was investigated on blastocyst of the PCOS experimental mouse model.

Materials and methods

30 NMRI female mice were equally divided into 3 groups: control, experimental [PCOS that was injected estradiol valerate (40 mg/kg)]. After 8 weeks, each group was hyper stimulated by PMSG and HCG. Vaginal plaque was checked, and mice were investigated 5 days after the test. Progesterone and estradiol levels were determined; α4, αv, β1 and β3 integrin genes and protein of blastocysts were examined by real time PCR method and immunohistochemistry, respectiv

SUBMITTER: Peyghambari F 

PROVIDER: S-EPMC4248147 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature