Alleviated mucosal and neuronal damage in a rat model of Crohn's disease.

ABSTRACT:

Aim

To establish a rat model suitable to investigate the repetitive relapsing inflammations (RRI) characteristic to Crohn's disease.

Methods

Colitis was induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS). RRI were mimicked by repeating administrations of TNBS. Tissue samples were taken from control, once, twice and three times treated rats from the inflamed and adjacent non-inflamed colonic segments at different timepoints during the acute intestinal inflammation. The means of the ulcerated area were measured to evaluate the macroscopic mucosal damage. The density of myenteric neurons was determined on whole mounts by HuC/HuD immunohistochemistry. Heme oxygenase-1 (HO-1) expression was evaluated by molecular biological techniques.

Results

TNBS-treated rats displayed

SUBMITTER: Talapka P 

PROVIDER: S-EPMC4248213 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature