Identification and molecular characterization of tissue-preferred rice genes and their upstream regularly sequences on a genome-wide level.

ABSTRACT:

Background

Gene upstream regularly sequences (URSs) can be used as one of the tools to annotate the biological functions of corresponding genes. In addition, tissue-preferred URSs are frequently used to drive the transgene expression exclusively in targeted tissues during plant transgenesis. Although many rice URSs have been molecularly characterized, it is still necessary and valuable to identify URSs that will benefit plant transformation and aid in analyzing gene function.

Results

In this study, we identified and characterized root-, seed-, leaf-, and panicle-preferred genes on a genome-wide level in rice. Subsequently, their expression patterns were confirmed through quantitative real-time RT-PCR (qRT-PCR) by randomly selecting 9candidate tissue-preferred genes. In addit

SUBMITTER: Jiang SY 

PROVIDER: S-EPMC4248441 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature