In situ serial Laue diffraction on a microfluidic crystallization device.

ABSTRACT: Renewed interest in room-temperature diffraction has been prompted by the desire to observe structural dynamics of proteins as they function. Serial crystallography, an experimental strategy that aggregates small pieces of data from a large uniform pool of crystals, has been demonstrated at synchrotrons and X-ray free-electron lasers. This work utilizes a microfluidic crystallization platform for serial Laue diffraction from macroscopic crystals and proposes that a collection of small slices of Laue data from many individual crystals is a realistic solution to the difficulties in dynamic studies of irreversible biochemical reactions.

SUBMITTER: Perry SL 

PROVIDER: S-EPMC4248567 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature