Development of novel filtering criteria to analyze RNA-sequencing data obtained from the murine ocular lens during embryogenesis.

ABSTRACT: Next-generation sequencing of the transcriptome (RNA-Seq) is a powerful method that allows for the quantitative determination of absolute gene expression, and can be used to investigate how these levels change in response to an experimental manipulation or disease condition. The sensitivity of this method allows one to analyze transcript levels of all expressed genes, including low abundance transcripts that encode important regulatory molecules, providing valuable insights into the global effects of experimental manipulations. However, this increased sensitivity can also make it challenging to ascertain which expression changes are biologically significant. Here, we describe a novel set of filtering criteria - based on biological insights and computational approaches - that were applied t

SUBMITTER: Manthey AL 

PROVIDER: S-EPMC4248573 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature