Immunologic characterization of a rhesus macaque H1N1 challenge model for candidate influenza virus vaccine assessment.

ABSTRACT: Despite the availability of annually formulated vaccines, influenza virus infection remains a worldwide public health burden. Therefore, it is important to develop preclinical challenge models that enable the evaluation of vaccine candidates while elucidating mechanisms of protection. Here, we report that naive rhesus macaques challenged with 2009 pandemic H1N1 (pH1N1) influenza virus do not develop observable clinical symptoms of disease but develop a subclinical biphasic fever on days 1 and 5 to 6 postchallenge. Whole blood microarray analysis further revealed that interferon activity was associated with fever. We then tested whether type I interferon activity in the blood is a correlate of vaccine efficacy. The animals immunized with candidate vaccines carrying hemagglutinin (HA) or nuc

SUBMITTER: Skinner JA 

PROVIDER: S-EPMC4248778 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature