Flocculation causes inhibitor tolerance in Saccharomyces cerevisiae for second-generation bioethanol production.

ABSTRACT: Yeast has long been considered the microorganism of choice for second-generation bioethanol production due to its fermentative capacity and ethanol tolerance. However, tolerance toward inhibitors derived from lignocellulosic materials is still an issue. Flocculating yeast strains often perform relatively well in inhibitory media, but inhibitor tolerance has never been clearly linked to the actual flocculation ability per se. In this study, variants of the flocculation gene FLO1 were transformed into the genome of the nonflocculating laboratory yeast strain Saccharomyces cerevisiae CEN.PK 113-7D. Three mutants with distinct differences in flocculation properties were isolated and characterized. The degree of flocculation and hydrophobicity of the cells were correlated to the length of the g

SUBMITTER: Westman JO 

PROVIDER: S-EPMC4249023 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature