Expression library immunization can confer protection against lethal challenge with African swine fever virus.

ABSTRACT:

Unlabelled

African swine fever is one of the most devastating pig diseases, against which there is no vaccine available. Recent work from our laboratory has demonstrated the protective potential of DNA vaccines encoding three African swine fever viral antigens (p54, p30, and the hemagglutinin extracellular domain) fused to ubiquitin. Partial protection was afforded in the absence of detectable antibodies prior to virus challenge, and survival correlated with the presence of a large number of hemagglutinin-specific CD8(+) T cells in blood. Aiming to demonstrate the presence of additional CD8(+) T-cell determinants with protective potential, an expression library containing more than 4,000 individual plasmid clones was constructed, each one randomly containing a Sau3AI restriction fr

SUBMITTER: Lacasta A 

PROVIDER: S-EPMC4249112 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature