Anterograde glycoprotein-dependent transport of newly generated rabies virus in dorsal root ganglion neurons.

ABSTRACT:

Unlabelled

Rabies virus (RABV) spread is widely accepted to occur only by retrograde axonal transport. However, examples of anterograde RABV spread in peripheral neurons such as dorsal root ganglion (DRG) neurons indicated a possible bidirectional transport by an uncharacterized mechanism. Here, we analyzed the axonal transport of fluorescence-labeled RABV in DRG neurons by live-cell microscopy. Both entry-related retrograde transport of RABV after infection at axon endings and postreplicative transport of newly formed virus were visualized in compartmentalized DRG neuron cultures. Whereas entry-related transport at 1.5 μm/s occurred only retrogradely, after 2 days of infection, multiple particles were observed in axons moving in both the anterograde and retrograde directions. The

SUBMITTER: Bauer A 

PROVIDER: S-EPMC4249153 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature