Routes of Acquisition of the Gut Microbiota of the Honey Bee Apis mellifera.

ABSTRACT: Studies of newly emerged Apis mellifera worker bees have demonstrated that their guts are colonized by a consistent core microbiota within several days of eclosure. We conducted experiments aimed at illuminating the transmission routes and spatiotemporal colonization dynamics of this microbiota. Experimental groups of newly emerged workers were maintained in cup cages and exposed to different potential transmission sources. Colonization patterns were evaluated using quantitative real-time PCR (qPCR) to assess community sizes and using deep sequencing of 16S rRNA gene amplicons to assess community composition. In addition, we monitored the establishment of the ileum and rectum communities within workers sampled over time from natural hive conditions. The study verified that workers initiall

SUBMITTER: Powell JE 

PROVIDER: S-EPMC4249178 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature