Involvement of stress-related genes polB and PA14_46880 in biofilm formation of Pseudomonas aeruginosa.

ABSTRACT: Chronic infections of Pseudomonas aeruginosa are generally established through production of biofilm. During biofilm formation, production of an extracellular matrix and establishment of a distinct bacterial phenotype make these infections difficult to eradicate. However, biofilm studies have been hampered by the fact that most assays utilize nonliving surfaces as biofilm attachment substrates. In an attempt to better understand the mechanisms behind P. aeruginosa biofilm formation, we performed a genetic screen to identify novel factors involved in biofilm formation on biotic and abiotic surfaces. We found that deletion of genes polB and PA14_46880 reduced biofilm formation significantly compared to that in the wild-type strain PA14 in an abiotic biofilm system. In a biotic biofilm model,

SUBMITTER: Alshalchi SA 

PROVIDER: S-EPMC4249315 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature