Online SERS detection of the 20 proteinogenic L-amino acids separated by capillary zone electrophoresis.

ABSTRACT: A sheath-flow surface-enhanced Raman scattering (SERS) detector is demonstrated to provide chemical information enabling identification of the 20 proteinogenic L-amino acids separated by capillary zone electrophoresis (CZE). Amino acids were used to illustrate the chemical specificity of SERS detection from structurally related molecules. Analysis of the SERS electropherograms obtained from the separation and sequential online detection of six groups of structurally related amino acids shows that our sheath-flow SERS detector is able to resolve the characteristic Raman bands attributed to the amine, carboxyl, and side chain constituents. The results demonstrate the chemical information available from our detector and also provide insight into the nature of the analyte interaction with the

SUBMITTER: Negri P 

PROVIDER: S-EPMC4249764 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature