The accuracy, feasibility and challenges of sequencing short tandem repeats using next-generation sequencing platforms.

ABSTRACT: To date we have little knowledge of how accurate next-generation sequencing (NGS) technologies are in sequencing repetitive sequences beyond known limitations to accurately sequence homopolymers. Only a handful of previous reports have evaluated the potential of NGS for sequencing short tandem repeats (microsatellites) and no empirical study has compared and evaluated the performance of more than one NGS platform with the same dataset. Here we examined yeast microsatellite variants from both long-read (454-sequencing) and short-read (Illumina) NGS platforms and compared these to data derived through Sanger sequencing. In addition, we investigated any locus-specific biases and differences that might have resulted from variability in microsatellite repeat number, repeat motif or type of muta

SUBMITTER: Zavodna M 

PROVIDER: S-EPMC4250034 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature