Targeting survival pathways to create infarct-spanning bridges of human embryonic stem cell-derived cardiomyocytes.

ABSTRACT:

Objective

Generating myocyte grafts that bridge across infarcts could maximize their functional impact and best utilize small numbers of stem cells. To date, however, graft survival within acute infarcts has not been feasible. To enhance intrainfarct graft viability, human embryonic stem cell-derived cardiomyocytes (hESC-CMs) were pretreated before implantation with cobalt protoporphyrin (CoPP), a pharmacologic inducer of cytoprotective heme oxygenase-1.

Methods

After preculturing with CoPP (vs phosphate-buffered saline), hESC-CMs were injected intramyocardially into acutely infarcted rat hearts, using directed injections to span the infarct. A further group received CoPP-pretreated hESC-CMs plus 4 weekly doses of systemic CoPP to prolong exposure to cytoprotectants. Two con

SUBMITTER: Luo J 

PROVIDER: S-EPMC4250416 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature