Composition of protein supplements used for human embryo culture.

ABSTRACT:

Purpose

To determine the composition of commercially available protein supplements for embryo culture media and test if differences in protein supplement composition are biologically relevant in a murine model.

Methods

Amino acid, organic acid, ion and metal content were determined for 6 protein supplements: recombinant human albumin (AlbIX), human serum albumin (HSA and Buminate), and three complex protein supplements (SSS, SPS, LGPS). To determine if differences in the composition of these supplements are biologically relevant, mouse one-cell embryos were collected and cultured for 120 hours in each protein supplement in Global media at 5 and 20 % oxygen in an EmbryoScope time-lapse incubator. The compositions of six protein supplements were analyzed for concentrations of

SUBMITTER: Morbeck DE 

PROVIDER: S-EPMC4250459 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature