Gene expression profile in rat small intestinal allografts after cold preservation/reperfusion.

ABSTRACT:

Aim

To determine the changes of gene expression profile in small intestinal allografts in rats after cold preservation/reperfusion, and to identify the genes relevant to cold preservation/reperfusion injury.

Methods

Heterotopic segmental small bowel transplantation was performed in six rats with a sham operation and they were used as controls. Total RNA was extracted from the allografts (experimental group) and normal intestines (control group) 1 h after cold preservation/reperfusion, and then purified to mRNA, which was then reversely transcribed to cDNA, and labeled with fluorescent Cy5-dUTP and Cy3-dUTP to prepare hybridization probes. The mixed probes were hybridized to the cDNA microarray. After high-stringent washing, the fluorescent signals on cDNA microarray chip wer

SUBMITTER: Wang SF 

PROVIDER: S-EPMC4250603 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature