Identification of differentially expressed genes after partial rat liver ischemia/reperfusion by suppression subtractive hybridization.

ABSTRACT:

Aim

To identify potential diagnostic target genes in early reperfusion periods following warm liver ischemia before irreversible liver damage occurs.

Methods

We used two strategies (SSH suppression subtractive hybridization and hybridization of cDNA arrays) to determine early changes in gene expression profiles in a rat model of partial WI/R, comparing postischemic and adjacent nonischemic liver lobes. Differential gene expression was verified (WI/R; 1 h/2 h) and analyzed in more detail after warm ischemia (1 h) in a reperfusion time kinetics (0, 1, 2 and 6 h) and compared to untreated livers by Northern blot hybridizations. Protein expression was examined on Western blots and by immunohistochemistry for four differentially expressed target genes (Hsp70, Hsp27, Gadd45a and I

SUBMITTER: Fallsehr C 

PROVIDER: S-EPMC4250677 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature