Expression and immunoactivity of chimeric particulate antigens of receptor binding site-core antigen of hepatitis B virus.

ABSTRACT:

Aim

To improve the immunogenicity of receptor binding site of hepatitis B virus (HBV) on preS1 antigen using HBV core antigen as an immuno-carrier.

Methods

One to 6 tandem copies of HBV preS1 (21-47) fragment were inserted into HBcAg at the sites of aa 78 and 82, and expressed in E.coli. ELISA, Western blot and animal immunization were used to analyze the antigenicity and immmunogenicity of purified particulate antigens. The ability to capture HBV by antibodies elicited by chimeric particles was detected with immuno-capture PCR.

Results

Recombinant antigens CI, CII, CIII carrying 1-3 copies of HBV preS1 (21-47) individually could form virus-like particles (VLPs), similar to HBcAg in morphology. But recombinant antigens carrying 4-6 copies of HBV preS1 (21-47) were poo

SUBMITTER: Yang HJ 

PROVIDER: S-EPMC4250797 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature