Bipolar/rod-shaped microglia are proliferating microglia with distinct M1/M2 phenotypes.

ABSTRACT: Microglia are generally considered the resident immune cells in the central nervous system (CNS) that regulate the primary events of neuroinflammatory responses. Microglia also play key roles in repair and neurodegeneration of the CNS after injury. Recent studies showed that trains of bipolar/rod-shaped microglia align end-to-end along the CNS injury site during the initial recovery phase. However, the cellular characteristics of bipolar/rod-shaped microglia remain largely unknown. Here, we established a highly reproducible in vitro culture model system to enrich and characterize bipolar/rod-shaped microglia by simply generating multiple scratches on a poly-d-lysine/laminin-coated culture dish. Trains of bipolar/rod-shaped microglia formed and aligned along the scratches in a manner that m

SUBMITTER: Tam WY 

PROVIDER: S-EPMC4250916 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature