Low-oxygen culture conditions extend the multipotent properties of human retinal progenitor cells.

ABSTRACT:

Purpose

Development of an effective cell-based therapy is highly dependent upon having a reproducible cell source suitable for transplantation. One potential source, isolated from the developing fetal neural retina, is the human retinal progenitor cell (hRPC). One limiting factor for the use of hRPCs is their in vitro expansion limit. As such, the aim of this study was to determine whether culturing hRPCs under 3% O2 would support their proliferative capacity while maintaining multipotency.

Methods

To determine the effect of low oxygen on the ability of hRPCs to self-renew, rates of proliferation and apoptosis, telomerase activity, and expression of proliferative, stemness, and differentiation markers were assessed for hRPCs cultured in 3% and 20% oxygen conditions.

Resul

SUBMITTER: Baranov PY 

PROVIDER: S-EPMC4251088 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature